Executive Summary

This draft plan describes a ten-year program to develop the scientific understanding needed to harness and develop natural and enhanced biogeochemical processes to bioremediate contaminated soils, sediments and groundwater at DOE facilities. The Office of Health and Environmental Research (OHER) developed this program plan, with advice and assistance from DOE's Office of Environmental Management (EM). The program builds on OHER's tradition of sponsoring fundamental research in the life and environmental sciences and was motivated by OHER's and Office of Energy Research's (OER's) commitment to supporting DOE's environmental management mission and the belief that bioremediation is an important part of the solution to DOE's environmental problems.

NATURAL AND ACCELERATED BIOREMEDIATION


Bioremediation is defined by the American Academy of Microbiology as "the use of living organisms to reduce or eliminate environmental hazards resulting from accumulations of toxic chemicals or other hazardous wastes" (Gibson and Sayler, 1992). The program addresses both natural bioremediation, which relies on naturally occurring microbial and plant processes, and accelerated bioremediation, which seeks to accelerate desirable processes through the addition of amendments (e.g., nutrients, electron acceptors) or microorganisms (including GEMs), or by manipulating physical, chemical, or hydrological processes. Bioremediation has been implemented successfully for degradation of petroleum hydrocarbons and, to a limited extent, degradation of explosives and chlorinated hydrocarbons, as well as for immobilization of toxic trace metals. However, the effectiveness of bioremediation cannot always be predicted reliably, due to numerous factors ranging from lack of basic scientific knowledge to engineering limitations. More importantly, few if any investigations have addressed bioremediation of the contaminants present at DOE sites, where mixtures containing chlorinated hydrocarbons, metals, radionuclides, PCBs, and inorganic contaminants are common. Finally, there is general agreement among the research community that field-based research is needed to realize the full potential of bioremediation.

THE SCIENTIFIC PROGRAM


The program was shaped by a team of scientists after a thorough review of recommendations for research published over the past five years and an analysis of related programs in DOE and other federal agencies. Several key themes guided the scientific approach:

o Fundamental research is required to advance our understanding of the biological, chemical, and physical processes important for natural and accelerated bioremediation.

o Integration of scientific ideas across disciplines is essential for the development of the knowledge needed to predict and optimize bioremediation rates and processes.

o Field research centers are the best vehicles for integrating research, identifying crucial research needs, and focusing the program on DOE's most significant problems.

o Access to R&D shared infrastructure will be required to advance measurement and diagnostic techniques.

o To realize the enormous potential that this program has to contribute to remediating DOE's sites, the goals must be identified clearly and used to focus the program.

o Ethical, legal, and social issues associated with bioremediation, especially with regard to genetically engineered organisms (such as GEM), must be identified and addressed immediately.

o Linkages to other, related programs must be established and maintained.

The key themes will be implemented through the seven interrelated program elements described below.

Biotransformation and Biodegradation

Fundamental research in microbiology to elucidate the mechanisms of biotransformation and biodegradation of complex contaminant mixtures.

Community Dynamics and Microbial Ecology

Fundamental research in ecological processes and interactions of biotic and abiotic components of ecosystems to understand their influence on the degradation, persistence, and toxicity of mixed contaminants.

Biomolecular Science and Engineering

Fundamental research in molecular and structural biology to enhance our undestanding of bioremediation and improve the efficacy of bioremedial organisms and identify novel remedial genes.

Biogeochemical Dynamics

Fundamental research in the dynamic relationships among in situ geochemical, geological, hydrological, and microbial processes.

Assessment

Fundamental research in measuring and validating the biological and geochemical processes of bioremediation.

Acceleration

Fundamental interdisciplinary research in flow and transport of nutrients and microorganisms, focused on developing effective methods for accelerating and optimizing bioremediation rates.

System Integration, Prediction, and Optimization

Fundamental research to develop conceptual and quantitative methods for describing community dynamics, biotransformation, biodegradation, and biogeochemical dynamics processes in complex geologic systems.

MANAGEMENT STRUCTURE AND IMPLEMENTATION


The program will be managed by OHER, with assistance from a program office. The program office will be responsible for day-to-day management, scientific coordination within and among program elements, scheduling of major activities at the test sites, communication and data base management, and facilitating access to the R&D shared infrastructure. Each field research center will have an on-site manager who will be responsible for obtaining permits, developing and implementing a site safety plan, day-to-day scheduling of field activities, and site operations. Instrument managers will be identified for major laboratory measurement facilities. Final management approval and oversight for all activities will be retained by OHER. The program will also be supported by an interagency steering committee to ensure coordination with complementary programs and identify opportunities for leveraging scientific and infrastructure investments.

Research projects will be carried out by a science team consisting of DOE laboratory scientists, academic investigators, researchers from other federal agencies, and industry researchers. The scientific program will be funded primarily by competitive proposals, regardless of the status of the principal investigator, be it university, DOE laboratory, or industry. The program will seek to get the best talent available to address the goals identified in the program elements.

The activities described above will be funded by OHER. As proposed by the team developing this program, the approximate total budget over the life of the ten-year program is $417M, of which 75% supports scientific effort and the remainder is divided equally among equipment and operating expenses.

The startup and implementation of the program will begin in FY96. FY96 activities will include: (1) broad announcement of the program to the research community and DOE sites, (2) selection of the scientific team leaders, (3) formation of the initial scientific team through a competitive process, (4) selection of the first field research center, (5) evaluation of R&D shared infrastructure requirements, (6) identification of ethical, legal, and social issues that must be addressed immediately, and (7) identification of the interagency steering committee members.

Two types of peer review will be incorporated into the program management plan programmatic peer review and project peer review. Programmatic peer review will be used to evaluate the overall effectiveness of the program and how well the program is achieving its goals. Project peer review will consist of conventional scientific peer review of individual projects proposed by the principal investigators of the program.

In addition to peer review, other performance measures will be used to evaluate whether the program is successful. These will include quantity and quality of new knowledge generated by the program, effectiveness of the interdisciplinary teams, timely accomplishments of programmatic goals, application of knowledge or techniques to bioremediation or to otherwise help solve DOE problems, and spin-off to other areas such as, pollution prevention, ecosystem studies, biomedical applications, ecotoxicity, and human genome studies.

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